Proteomics Unit
Mission
The Proteomics Unit's main objective is to promote scientific research and technological development in the field of proteomics, offering research groups, hospitals and companies various proteomics services, advice on their projects, as well as seminars and training courses.
Description
In the Proteomics Unit, not only are isolated proteins identified. The techniques offered enable the proteins that make up the proteome to be identified on a large scale. Quantitative proteomics studies can be carried out to compare the relative abundance of proteins in different samples. In addition, studies of protein interactions with other proteins and studies of post-translational modifications such as phosphorylations can be performed. Furthermore, targeted Proteomics services are offered, enabling biomarkers to be sought and validated in complex biological and clinical samples.
Services offered by the Proteomics Unit
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Mass Spectrometry |
Determination of the molecular masses of proteins. Protein identification by peptide mass fingerprinting. (MS) Protein identification by peptide fragmentation. (MS/MS) Peptide sequencing. |
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Liquid chromatography-coupled mass spectrometry |
Analysis of highly complex protein mixtures. Analysis of subproteomes. Study of cell surface proteins (surfome) |
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Protein separation |
One-dimensional electrophoresis, SDS-PAGE. And two-dimensional electrophoresis (2D-PAGE) Preparative liquid-phase isoelectric focusing. Separation of protein complexes (Blue-Native) Immunoproteome analysis. |
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Peptide separation |
Reverse-phase chromatography Ion-exchange chromatography Preparative liquid-phase isoelectric focusing. |
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Study of differential expression |
Gel-based quantitative proteomics: DIGE fluorophore labelling. Gel-free quantitative proteomics: iTRAQ isotopic labelling, SILAC metabolic labelling Label-free quantitative proteomics |
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Study of post-translational modifications |
Phosphoproteomics. Other modifications: acetylation, ubiquitination… |
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Targeted proteomics studies |
MRM analysis. |
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Others |
Transfer to PVDF membranes.Mass spectrometry |
Installation
The Proteomics Unit is a unit of the Centre for Research Assistance (CAI) for Genomics and Proteomics at the Complutense University of Madrid, located in the Faculty of Pharmacy of the Complutense University of Madrid (UCM). It belongs to the network of Laboratories of the Community of Madrid (LAB.185).
Technology
- Q-Exactive HF mass spectrometer (Thermo Finnigan).
- QTRAP 5500 mass spectrometer (AB SCIEX).
- 4800 Proteomics Analyser mass spectrometer with TOF/TOF (AB SCIEX). LTQ segmented linear ion-trap mass spectrometer (Thermo Finnigan).
- ETTAN LC capillary nano-liquid chromatography system (GE).
- Typhoon 9400 fluorescence scanner (GE).
- EttanTM system for two-dimensional electrophoresis (GE). OFFGEL liquid isoelectric focusing fractionator (Agilent).
People
Dr Concha Gil García
Professor of Microbiology at the Universidad Complutense de Madrid. Researcher in the Department of Microbiology and scientific director of the UCM-Madrid Science Park Proteomics Unit. Vice-President of the Spanish Proteomics Society.
Technical Staff:
María Luisa Hernáez Sánchez, BSc in Pharmacy from the Universidad Complutense de Madrid, Master's degree in Biotechnology from the Universidad Complutense de Madrid and technical director of the Proteomics Unit.
Lola Gutiérrez Blázquez, BSc in Biological Sciences from the Universidad Complutense de Madrid, Master's degree in Biotechnology from the Universidad Complutense de Madrid
Felipe Clemente Velarde, PhD in Biochemistry from the Universidad Autónoma de Madrid. Inés Zapico, BSc in Chemical Sciences from the Universidad de Oviedo.
Enrique Pérez, Master's degree in Bioinformatics from the Universidad de Murcia.
Unit Documentation Guide
The unit has all procedures, technical instructions and records in accordance with the ISO UNE-EN ISO 9001:2008 standard obtained in 2010.
Contact persons
Dra. Concha Gil García
Scientific manager
E-mail: conchagil(ELIMINAR)@ucm.es
María Luisa Hernáez Sánchez
Technical manager
E-mail: mlhernae(ELIMINAR)@ucm.es
Proteomics Unit Pza. Ramón y Cajal, s/n
Facultad de Farmacia - UCM 28040 - Madrid
Tel: (+34) 91 394 16 13
Fax: (+34) 91 394 17 45
cai.proteomica(ELIMINAR)@pas.ucm.es