Translational Research Laboratory in Cardiology
Mission
The Translational Research Laboratory in Cardiology, formerly the Laboratory of Bioartificial Organs and Matrices, is an infrastructure of the Research Institute of the Hospital Gregorio Marañón that forms part of the Organ Platform, whose Scientific Director is Prof. Fernández-Avilés, focusing on the field of Regenerative Medicine.
Description
The laboratory began its work focused on obtaining decellularised matrices from animal and human organs, with the aim of subsequently recellularising them with different cell types, thereby achieving viable matrices that could serve as replacement constructs for diseased organs and not be rejected upon transplantation. Throughout its history, it has strengthened these lines of research in Advanced Therapies with others linked to research in cardiology. At present, it is a Research Support Service that combines basic, preclinical and clinical research focused on understanding the specific processes at the molecular, cellular and organ levels that underlie the broad spectrum of cardiovascular diseases. The laboratory has implemented various cutting-edge technologies that support translational research in Cardiology and other disciplines within our hospital, as well as other nationally and internationally renowned research groups. These include: Rec different?ellularisation with Bioreactors, 3D Bioprinting, Basic Electrophysiology, Genomics, Transcriptomics, Metagenomics, etc..
The SAI is managed under the IISGM’s ISO 9001: 2015 quality system, which enables all projects to be carried out reproducibly and with verified traceability, in order to accelerate clinical translation.
Services offered
The HIV HGM Biobank offers the following services:
- Organ decellularization
- Study of ventricular mechanics in intact hearts and decellularized matrices
- Histology of samples obtained from the different matrices
- Cellular and Tissue Electrophysiology Programme
- Static recellularization of the different decellularized matrices
- Recellularization Programme using bioreactors.
- Culture of cells from animal tissue: Mesenchymal cells, iPSCs, cardiomyocytes, cardiac progenitors, etc..
- Culture of human cells:
- Stromal vascular fraction of adipose tissue (SVF)
- Bone marrow mononuclear cells
- Bone marrow mesenchymal cells
- Adipose tissue mesenchymal cells
- Cardiac progenitors (CPCs, CDCs, ...)
- hiPSC generation
- Differentiation of hiPSCs into cardiomyocytes
- Maintenance of different cell lines
- Studies of genetic variants, transcriptomics and metagenomics.
- Teaching and training platform
Technology
Histology Programme
- Thermo Scientific Microm HM525-U model cryostat
- Thermo Scientific Microm STP120-2 model automatic carousel-type tissue processor.
- Thermo Scientific Microm EC350 model modular embedding centre
- Selecta Termofin model histology bath, code 3000459
- Thermo Scientific Microm HM325 model microtome
- Selecta INCUBAT model culture incubator
- Leica BOND III for automatic IHC and ISH staining
- Leica DMD108 Histology Station
- Leica ICC50 HD direct-light microscope
Culture and cryopreservation programme
- Nuaire CO2 incubators. Nu-4850-E and Nuaire Nu-4950-E with CO2 and O2 control
- Nuaire laminar-flow biosafety cabinets, Nu-437-400-E.
- Safefast Elite model isolator
- 37ºC (Thermarks) and 22 ºC (Memment) incubators
- Captair Flex AM321 extraction cabinet for toxic substances
- DMI3000 inverted fluorescence microscope with built-in camera.
- Taylor-Wharton model liquid nitrogen tank, Cryocon M505K
- Taylor-Wharton L5750 liquid nitrogen container–milk churn
- -80ºC freezers, Profiline and Innova U-360 models.
Cellular and tissue electrophysiology programme
- Langendorf medium-sized heart system (pig-human)
- Electrical mapping system for Langendorf and cultures
- Optical mapping system for Langendorf
- Optical mapping system for cultures or tissues
- Electrical stimulation system
- Decellularisation Programme
- Marterflex 7524-45 peristaltic pumps
- Patch-clamp set
Genetics, genomics and transcriptomics programme
- Basic molecular biology equipment
- Real-time quantitative PCR (RT-PCR) equipment (CFX BioRad)
- Gel imaging and analysis software equipment
- Trans Blot Turbo gel-to-membrane transfer equipment (BioRad)
- ImageQuant LAS 500 equipment (GE Healhcare)
- Server for editing and analysing large-scale sequencing data
Specific techniques sections
- Flow cytometry with a 3-laser MacsQuant cytometer
- Cell purification with an AutoMACS Pro immunomagnetic separator.
- Rokit in vivo 3D bioprinter (Palex Medical)
Recellularisation programmes / bioreactors
- Bioreactors for human and animal organs
- Bioreactors with electromechanical stimulation
- Various static and dynamic cell-seeding systems.
Installation
The Translational Research Laboratory in Cardiology, formerly the Bioartificial Organs and Matrices Laboratory, is located on floor -1 of the Hospital Materno Infantil. It occupies an area of 275 m2 divided into different, clearly defined work areas: Histology Area, Cell Culture Area, Electrophysiology Area and Genetics, Genomics and Transcriptomics Area.
Contact
Dr Javier Bermejo Thomas
Scientific Director
Telephone: 91 426 58 82
E-mail: javier.bermejo(ELIMINAR)@salud.madrid.org
Dr Mª Eugenia Fernández Santos
Technical Director
Telephone: 91 529 0520-0521; Mobile: 657 65 0527
E-mail: mariuge(ELIMINAR)@fibhgm.org
E-mail: cardiologiatraslacional(ELIMINAR)@iisgm.com
Hospital GU Gregorio Marañón.
Mother and Child Building. Floor -1
C/ O’Donnell No. 48-52; 28009-Madrid.